Hotspot

rpl32-trnL-UAG

SSC · 108284-109001 · 718 bp

Back to Dendrocalamus

Pi 0.00453
MarkerSeek score 41.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
718
variable_sites
11
pi
0.00453
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 802-803 bp 1.0 79.1
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 801-802 bp 1.0 79.1
rpl32-trnL-UAG_p3 AAGATCATTTTCCAGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 839-840 bp 1.0 78.9
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 795-796 bp 1.0 78.6
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 794-795 bp 1.0 78.6