Hotspot

rpl22-rps19

LSC · 83051-83121 · 71 bp

Back to Dendrocalamus

Pi 0.00367

MarkerSeek score 48.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 3
pi: 0.00367
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGAGTTTCCTTAGCCCACTC	CAAATTGGGGGAATTCGTGC	55.0	50.0	58.0	58.0	216-223 bp	1.0	83.5
rpl22-rps19_p2	CGAGTTTCCTTAGCCCACTC	GCAAATTGGGGGAATTCGTG	55.0	50.0	58.0	58.0	217-224 bp	1.0	83.5
rpl22-rps19_p3	TCCTTGCGAGTTTCCTTAGC	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	222-229 bp	1.0	82.7
rpl22-rps19_p4	TCCTTGCGAGTTTCCTTAGC	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	223-230 bp	1.0	82.7
rpl22-rps19_p5	CGAGTTTCCTTAGCCCACTC	ATGGTAGGTCGCAAATTGGG	55.0	50.0	58.0	58.2	227-234 bp	1.0	81.5