Hotspot

trnN-GUU-rpl32

SSC · 109589-111730 · 2142 bp

Back to Dendrobium

Pi 0.0403
MarkerSeek score 76.80
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
2142
variable_sites
624
pi
0.0403
unique_haplotype_count
69
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTACGTTTTT 50.0 27.3 58.1 52.0 583-2372 bp 0.805556 44.2
trnN-GUU-rpl32_p2 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTACGTTTT 50.0 27.3 58.1 52.0 584-2373 bp 0.805556 44.2
trnN-GUU-rpl32_p3 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTACGTTT 50.0 27.3 58.1 52.0 585-2374 bp 0.805556 44.2
trnN-GUU-rpl32_p4 ACGACCAGTCAGTTAACAGC TTTGACATAGAAGTACGTTTTT 50.0 27.3 57.8 52.0 589-2378 bp 0.805556 44.2
trnN-GUU-rpl32_p5 ACGACCAGTCAGTTAACAGC TTTTGACATAGAAGTACGTTTT 50.0 27.3 57.8 52.0 590-2379 bp 0.805556 44.2