Hotspot

3'rps12-trnV-GAC

IRb · 99194-101150 · 1957 bp

Back to Dendrobium

Pi 0.0093
MarkerSeek score 77.40
Primer available yes
Label 3'rps12-trnV-GAC

Feature Metadata

label_name
3'rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1957
variable_sites
248
pi
0.0093
unique_haplotype_count
65
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
3'rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1811-2185 bp 1.0 88.6
3'rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1815-2189 bp 1.0 88.6
3'rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1810-2184 bp 1.0 88.6
3'rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1813-2187 bp 1.0 87.7
3'rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1796-2170 bp 0.930556 84.4