Hotspot

clpP-psbB

LSC · 71190-71898 · 709 bp

Back to Dendrobium

Pi 0.0456

MarkerSeek score 82.50

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 280
pi: 0.0456
unique_haplotype_count: 69
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	702-971 bp	1.0	90.1
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	697-966 bp	0.986111	89.4
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	648-917 bp	1.0	88.8
clpP-psbB_p4	CGCACTATACGTCAACCCAA	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	705-974 bp	1.0	88.2
clpP-psbB_p5	TATACGTCAACCCAAGCTGC	ACACGATACCAAGGCAAACC	50.0	50.0	58.0	58.5	700-969 bp	0.986111	87.5