Hotspot

rpl20-5'rps12

LSC · 67935-68712 · 778 bp

Back to Dendrobium

Pi 0.013

MarkerSeek score 78.20

Primer available yes

Label rpl20-5'rps12

Feature Metadata

label_name: rpl20-5'rps12
feature_type: igs
region: LSC
length_bp: 778
variable_sites: 115
pi: 0.013
unique_haplotype_count: 61
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-5'rps12_p1	GCCCCTATAAAGGTTGATGCA	GAAATCTCCCGCTCTTCGAG	47.6	55.0	58.1	58.2	798-928 bp	1.0	82.3
rpl20-5'rps12_p2	GCCCCTATAAAGGTTGATGCA	TAGAAACGCAAGACAGCCAA	47.6	45.0	58.1	57.8	831-961 bp	1.0	81.6
rpl20-5'rps12_p3	GCCCCTATAAAGGTTGATGCA	TTAGAAACGCAAGACAGCCA	47.6	45.0	58.1	57.8	832-962 bp	1.0	81.6
rpl20-5'rps12_p4	AGCCCCTATAAAGGTTGATGC	GAAATCTCCCGCTCTTCGAG	47.6	55.0	57.8	58.2	799-929 bp	1.0	80.9
rpl20-5'rps12_p5	GCCCCTATAAAGGTTGATGCA	AGAAACGCAAGACAGCCAAT	47.6	45.0	58.1	58.4	830-960 bp	1.0	80.4