Hotspot

trnF-GAA-trnV-UAC

LSC · 48493-49893 · 1401 bp

Back to Dendrobium

Pi 0.0352

MarkerSeek score 82.90

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1401
variable_sites: 730
pi: 0.0352
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	981-2997 bp	0.722222	78.9
trnF-GAA-trnV-UAC_p2	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1024-2101 bp	0.708333	77.1
trnF-GAA-trnV-UAC_p3	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1023-2100 bp	0.708333	77.1
trnF-GAA-trnV-UAC_p4	CTCTGCTAGGATGATGCACG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	1123-2124 bp	0.652778	74.8
trnF-GAA-trnV-UAC_p5	GCTAGGATGATGCACGGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1530-1971 bp	0.069444	52.8