Hotspot

trnL-UAA-trnL-UAA

LSC · 47175-47976 · 802 bp

Back to Dendrobium

Pi 0.0382

MarkerSeek score 77.10

Primer available yes

Label trnL-UAA-trnL-UAA

Feature Metadata

label_name: trnL-UAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 255
pi: 0.0382
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnL-UAA_p1	GGCGAAATTGGTAGACGCTA	AGCGAGGAAGTAAAATGGGC	50.0	50.0	58.1	58.2	544-976 bp	1.0	89.5
trnL-UAA-trnL-UAA_p2	GGCGAAATTGGTAGACGCTA	GCGAGGAAGTAAAATGGGCT	50.0	50.0	58.1	58.2	543-975 bp	1.0	89.5
trnL-UAA-trnL-UAA_p3	GGGGATATGGCGAAATTGGT	AGCGAGGAAGTAAAATGGGC	50.0	50.0	58.0	58.2	552-984 bp	0.986111	89.4
trnL-UAA-trnL-UAA_p4	GGGGATATGGCGAAATTGGT	GCGAGGAAGTAAAATGGGCT	50.0	50.0	58.0	58.2	551-983 bp	0.986111	89.4
trnL-UAA-trnL-UAA_p5	GGGGATATGGCGAAATTGGT	GGACTTGAACCCTCACGATT	50.0	50.0	58.0	57.5	514-946 bp	0.972222	87.1