Hotspot

trnE-UUC-trnT-GGU

LSC · 31537-32078 · 542 bp

Back to Dendrobium

Pi 0.037

MarkerSeek score 78.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 199
pi: 0.037
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCGTAGTTCACTACCCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	559-1327 bp	1.0	90.7
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	524-1292 bp	1.0	90.6
trnE-UUC-trnT-GGU_p3	TCGTAGTTCACTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.2	58.1	538-1306 bp	0.930556	87.5
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	503-1271 bp	0.930556	87.4
trnE-UUC-trnT-GGU_p5	TCGTAGTTCACTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.2	58.1	535-1303 bp	0.930556	87.3