Hotspot

petN-psbM

LSC · 29171-29943 · 773 bp

Back to Dendrobium

Pi 0.0359

MarkerSeek score 82.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 259
pi: 0.0359
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.2	57.9	883-1088 bp	0.972222	88.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	822-1027 bp	1.0	88.8
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	874-1079 bp	1.0	88.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	875-1080 bp	1.0	87.8
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	CTTGCGCTTATTGCTACTGC	55.0	50.0	57.5	57.9	826-1031 bp	0.972222	86.2