Hotspot

atpH-atpI

LSC · 13983-14713 · 731 bp

Back to Dendrobium

Pi 0.0196

MarkerSeek score 81.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 197
pi: 0.0196
unique_haplotype_count: 62
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	50.0	45.0	57.9	57.9	609-888 bp	1.0	90.4
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	50.0	45.0	58.1	57.9	660-939 bp	0.986111	89.9
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	45.0	45.0	57.9	57.9	588-867 bp	1.0	89.8
atpH-atpI_p4	TCCGATAGAAGCAAGCCCTA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	585-864 bp	0.986111	89.4
atpH-atpI_p5	CGCAATACCTTCTACGGCTT	CGAATCCATGGAGGGTCATC	50.0	55.0	58.1	57.8	636-915 bp	0.972222	89.0