Hotspot

psbK-psbI

LSC · 7539-8056 · 518 bp

Back to Dendrobium

Pi 0.0346

MarkerSeek score 79.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 153
pi: 0.0346
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	520-638 bp	1.0	91.0
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	521-639 bp	1.0	91.0
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	522-640 bp	1.0	89.2
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.3	58.1	517-635 bp	1.0	89.0
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.3	58.1	518-636 bp	1.0	89.0