Hotspot

rps16-trnQ-UUG

LSC · 5908-6911 · 1004 bp

Back to Dendrobium

Pi 0.0383

MarkerSeek score 82.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 415
pi: 0.0383
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TCATGTCCTTCAAGTCGCAC	TACTCGGAGGTTCGAATCCT	50.0	50.0	58.2	57.6	934-1195 bp	1.0	86.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	923-1184 bp	0.986111	86.9
rps16-trnQ-UUG_p3	TCATGTCCTTCAAGTCGCAC	ACTCGGAGGTTCGAATCCTT	50.0	50.0	58.2	58.4	933-1194 bp	1.0	86.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	ACTCGGAGGTTCGAATCCTT	45.0	50.0	58.1	58.4	922-1183 bp	0.986111	86.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGTTACTC	45.0	55.0	58.1	58.5	938-1199 bp	0.972222	86.0