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rps8-rpl14

LSC · 80917-81160 · 244 bp

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Pi 0.0203

MarkerSeek score 69.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 28
pi: 0.0203
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	50.0	50.0	57.7	58.0	334-371 bp	0.923077	83.0
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	GCTCGGGAATTGAGACAGTT	50.0	50.0	57.7	57.9	311-348 bp	0.923077	82.6
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	57.7	58.1	332-369 bp	0.923077	82.2
rps8-rpl14_p4	AGCAATAGTGTCCCTACCCA	AACTCGAGTTTTTGGTGCGA	50.0	45.0	57.7	58.3	333-370 bp	0.923077	80.4
rps8-rpl14_p5	CAGCAATAGTGTCCCTACCC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	57.4	58.0	335-372 bp	0.923077	80.1