Hotspot

trnP-UGG-psaJ

LSC · 66663-67350 · 688 bp

Back to Delphinium

Pi 0.0271

MarkerSeek score 80.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 107
pi: 0.0271
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	AATGTCAACGCATCTGGGAA	50.0	45.0	58.1	57.8	80-903 bp	1.0	89.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	AATGTCAACGCATCTGGGAA	50.0	45.0	58.1	57.8	80-902 bp	1.0	89.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	AATGTCAACGCATCTGGGAA	50.0	45.0	58.1	57.8	80-914 bp	1.0	89.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	AATGTCAACGCATCTGGGAA	50.0	45.0	58.1	57.8	80-913 bp	1.0	89.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	AATGTCAACGCATCTGGGAA	50.0	45.0	58.1	57.8	82-916 bp	1.0	89.5