Hotspot

trnK-UUU-trnQ-UUG

LSC · 4290-5049 · 760 bp

Back to Delphinium

Pi 0.0203

MarkerSeek score 74.90

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 85
pi: 0.0203
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	386-823 bp	1.0	90.0
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	385-822 bp	1.0	90.0
trnK-UUU-trnQ-UUG_p3	TCTAGCCGCACTTAAAAGCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	400-837 bp	1.0	89.3
trnK-UUU-trnQ-UUG_p4	CCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	395-832 bp	1.0	89.1
trnK-UUU-trnQ-UUG_p5	AAGCCGAGTACTCTACCGTT	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	389-826 bp	1.0	89.0