Hotspot

ycf4

LSC · 63097-63651 · 555 bp

Back to Dehaasia

Pi 0.0036

MarkerSeek score 59.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 2
pi: 0.0036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TGAGAGTTACTTCGGGAGCA	ACGTTCCGAAGAAGCTTCAG	50.0	50.0	58.1	58.2	736 bp	1.0	77.8
ycf4_p2	TGAGAGTTACTTCGGGAGCA	CGTTCCGAAGAAGCTTCAGT	50.0	50.0	58.1	58.2	735 bp	1.0	77.8
ycf4_p3	TGAGAGTTACTTCGGGAGCA	AACGTTCCGAAGAAGCTTCA	50.0	45.0	58.1	57.8	737 bp	1.0	77.7
ycf4_p4	TGAGAGTTACTTCGGGAGCA	TGAACGTTCCGAAGAAGCTT	50.0	45.0	58.1	57.8	739 bp	1.0	77.7
ycf4_p5	TGAGAGTTACTTCGGGAGCA	ATGAACGTTCCGAAGAAGCT	50.0	45.0	58.1	57.5	740 bp	1.0	75.9