Hotspot

rpl2

LSC · 87958-89463 · 1506 bp

Back to Dehaasia

Pi 0.00398

MarkerSeek score 47.50

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 6
pi: 0.00398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GGAATGCCCACGAATCTGAT	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2614-2620 bp	1.0	80.1
rpl2_p2	ATAATGGTAGATGCCCGGGA	GAATGGTTAAAGCGCCCAAC	50.0	50.0	58.0	58.0	2254-2260 bp	1.0	80.0
rpl2_p3	GGAATGCCCACGAATCTGAT	ATACTGGAACTCGTGGGGAA	50.0	50.0	58.0	58.1	2415-2421 bp	1.0	79.8
rpl2_p4	GGAATGCCCACGAATCTGAT	ACGGGGACATTCAATAAGGC	50.0	50.0	58.0	57.9	2547-2553 bp	1.0	79.7
rpl2_p5	ATAATGGTAGATGCCCGGGA	ACGGGGACATTCAATAAGGC	50.0	50.0	58.0	57.9	2187-2193 bp	1.0	79.7