Hotspot

rbcL

LSC · 58325-59752 · 1428 bp

Back to Dehaasia

Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 2
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCGAGTAGACCTTGTTGTTGT	TGGGCCGAGTTTAATTGCAA	42.9	45.0	56.9	58.4	1532 bp	1.0	60.4
rbcL_p2	TCGAGTAGACCTTGTTGTTGT	TTGGGCCGAGTTTAATTGCA	42.9	45.0	56.9	58.4	1533 bp	1.0	60.4
rbcL_p3	TCGAGTAGACCTTGTTGTTGT	GGGAACGAACGGGAATTACT	42.9	50.0	56.9	57.6	1508 bp	1.0	60.3
rbcL_p4	CGAGTAGACCTTGTTGTTGTG	TGGGCCGAGTTTAATTGCAA	47.6	45.0	56.9	58.4	1531 bp	1.0	60.0
rbcL_p5	CGAGTAGACCTTGTTGTTGTG	TTGGGCCGAGTTTAATTGCA	47.6	45.0	56.9	58.4	1532 bp	1.0	60.0