Hotspot

ndhK

LSC · 51551-52228 · 678 bp

Back to Dehaasia

Pi 0.00147

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 1
pi: 0.00147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ATGAACGACCCTGCATCTTC	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	884 bp	1.0	79.2
ndhK_p2	GATGAACGACCCTGCATCTT	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	885 bp	1.0	79.2
ndhK_p3	GAACGACCCTGCATCTTCTT	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.9	58.1	882 bp	1.0	78.6
ndhK_p4	ATGAACGACCCTGCATCTTC	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.0	57.8	897 bp	1.0	78.3
ndhK_p5	GATGAACGACCCTGCATCTT	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.0	57.8	898 bp	1.0	78.3