Hotspot

rpl32-trnL-UAG

SSC · 117703-119077 · 1375 bp

Back to Dehaasia

Pi 0.00364
MarkerSeek score 46.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1375
variable_sites
5
pi
0.00364
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTGGACTCTATCTCCGAGCT ACTGGGATTGTAGAGCAGGT 55.0 50.0 58.0 58.0 2670 bp 1.0 79.8
rpl32-trnL-UAG_p2 GTGGACTCTATCTCCGAGCT TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.0 57.9 2139 bp 1.0 79.1
rpl32-trnL-UAG_p3 AGTAGATCTCGCCCGTAGAC ACTGGGATTGTAGAGCAGGT 55.0 50.0 58.1 58.0 2475 bp 1.0 79.0
rpl32-trnL-UAG_p4 GTGGACTCTATCTCCGAGCT CGCTTAACGGCAAATGTCTC 55.0 50.0 58.0 57.8 2475 bp 1.0 78.9
rpl32-trnL-UAG_p5 GTGGACTCTATCTCCGAGCT GCTTAACGGCAAATGTCTCG 55.0 50.0 58.0 57.8 2474 bp 1.0 78.9