Hotspot

trnC-GCA-petN

LSC · 29063-30119 · 1057 bp

Back to Dehaasia

Pi 0.00189

MarkerSeek score 59.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 2
pi: 0.00189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1169-1174 bp	1.0	78.5
trnC-GCA-petN_p2	CTGCAAATCCTTTCTCCCCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1125-1130 bp	1.0	77.2
trnC-GCA-petN_p3	TGCAAATCCTTTCTCCCCAG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1124-1129 bp	1.0	77.2
trnC-GCA-petN_p4	ACTGCAAATCCTTTCTCCCC	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1126-1131 bp	1.0	77.2
trnC-GCA-petN_p5	GCAAATCCTTTCTCCCCAGT	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1123-1128 bp	1.0	77.2