Hotspot

rpoB-trnC-GCA

LSC · 27880-28991 · 1112 bp

Back to Dehaasia

Pi 0.0045

MarkerSeek score 61.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 5
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	TTGTTAATCAGGCGACACCC	50.0	50.0	57.3	58.2	1218-1224 bp	1.0	73.5
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	CTGGGGAGAAAGGATTTGCA	50.0	50.0	57.3	57.8	1189-1195 bp	1.0	73.3
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	TGGGGAGAAAGGATTTGCAG	50.0	50.0	57.3	57.8	1188-1194 bp	1.0	73.3
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	AGAAAGGATTTGCAGTCCCC	50.0	50.0	57.3	57.8	1183-1189 bp	1.0	73.2
rpoB-trnC-GCA_p5	ATTCCCTCATTTCCATCCCG	GGGGAGAAAGGATTTGCAGT	50.0	50.0	57.3	57.8	1187-1193 bp	1.0	73.2