Hotspot

trnS-GCU-trnG-UCC

LSC · 9510-10336 · 827 bp

Back to Dehaasia

Pi 0.00363

MarkerSeek score 59.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 3
pi: 0.00363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCACT	45.0	42.9	58.0	58.2	901 bp	1.0	70.1
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	ACGAATCACGCTTTTACCAC	45.0	45.0	58.0	56.8	901 bp	1.0	70.0
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AACGAATCACGCTTTTACCAC	45.0	42.9	58.0	57.5	902 bp	1.0	67.7
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	CGAATCACGCTTTTACCACT	45.0	45.0	58.0	56.5	900 bp	1.0	67.7
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACGCTTTTACCACT	50.0	42.9	57.6	58.2	887 bp	1.0	67.1