Hotspot

rpl16

LSC · 82521-83954 · 1434 bp

Back to Debregeasia

Pi 0.0014

MarkerSeek score 60.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 3
pi: 0.0014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCAAAAGGCGACGTAAAAA	TGTCTTTTAACTCAAAACAGAA	42.1	27.3	55.3	52.1	1619-1627 bp	1.0	40.7
rpl16_p2	GCAAAAGGCGACGTAAAAA	TTGTCTTTTAACTCAAAACAGA	42.1	27.3	55.3	52.1	1620-1628 bp	1.0	40.7
rpl16_p3	TTCGTCCGAAAAACCTTTTT	TGTCTTTTAACTCAAAACAGAA	35.0	27.3	54.3	52.1	1548-1556 bp	1.0	40.7
rpl16_p4	TTCGTCCGAAAAACCTTTTT	TTGTCTTTTAACTCAAAACAGA	35.0	27.3	54.3	52.1	1549-1557 bp	1.0	40.7
rpl16_p5	TTTCGTCCGAAAAACCTTTT	TGTCTTTTAACTCAAAACAGAA	35.0	27.3	54.3	52.1	1549-1557 bp	1.0	40.7