Hotspot

rbcL

LSC · 55982-57430 · 1449 bp

Back to Debregeasia

Pi 0.0023

MarkerSeek score 60.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 5
pi: 0.0023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAACCGTTCTGATCAGTTG	TCTTGTATTCGGCTCAATCCT	50.0	42.9	56.9	56.8	1668 bp	1.0	53.6
rbcL_p2	CGAACCGTTCTGATCAGTTGA	TCTTGTATTCGGCTCAATCCT	47.6	42.9	58.3	56.8	1668 bp	1.0	52.1
rbcL_p3	CGAACCGTTCTGATCAGTTG	TCTTGTATTCGGCTCAATCCTT	50.0	40.9	56.9	57.5	1668 bp	1.0	51.2
rbcL_p4	CGAACCGTTCTGATCAGTTGA	TCTTGTATTCGGCTCAATCCTT	47.6	40.9	58.3	57.5	1668 bp	1.0	49.7
rbcL_p5	CGAACCGTTCTGATCAGTTG	ATCTTGTATTCGGCTCAATCCT	50.0	40.9	56.9	57.3	1669 bp	1.0	49.5