Hotspot

rps15-ycf1

SSC · 125230-125631 · 402 bp

Back to Debregeasia

Pi 0.00829

MarkerSeek score 47.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 402
variable_sites: 5
pi: 0.00829
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACAACAGACCCCTTCTTTTCT	ACAATGGCAGTCGTTTCAGT	42.9	45.0	57.0	58.0	544 bp	1.0	64.8
rps15-ycf1_p2	ACAACAGACCCCTTCTTTTCT	CCAACAATGGCAGTCGTTTC	42.9	50.0	57.0	57.9	547 bp	1.0	64.7
rps15-ycf1_p3	ACAACAGACCCCTTCTTTTCT	TGGCAGTCGTTTCAGTATGG	42.9	50.0	57.0	57.9	540 bp	1.0	64.4
rps15-ycf1_p4	ACAACAGACCCCTTCTTTTCT	GGCAGTCGTTTCAGTATGGT	42.9	50.0	57.0	57.9	539 bp	1.0	64.4
rps15-ycf1_p5	ACAACAGACCCCTTCTTTTCTT	ACAATGGCAGTCGTTTCAGT	40.9	45.0	57.7	58.0	544 bp	1.0	62.5