Hotspot

ndhD-psaC

SSC · 118670-118775 · 106 bp

Back to Debregeasia

Pi 0.022

MarkerSeek score 67.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 3
pi: 0.022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	TGTGGCATGAAACAACTCGA	45.0	45.0	57.3	58.0	229-230 bp	1.0	75.8
ndhD-psaC_p2	GAACCCGCAAATATTGGCAA	AGAGGACTGTGTGGGTTGTA	45.0	50.0	57.3	57.9	300-301 bp	1.0	75.0
ndhD-psaC_p3	GAACCCGCAAATATTGGCAA	TGTGAATCTGCCTGTCCAAC	45.0	50.0	57.3	58.1	275-276 bp	1.0	75.0
ndhD-psaC_p4	AGGAACCCGCAAATATTGGC	TGTGGCATGAAACAACTCGA	50.0	45.0	58.9	58.0	231-232 bp	1.0	74.0
ndhD-psaC_p5	AGGAACCCGCAAATATTGGC	AGAGGACTGTGTGGGTTGTA	50.0	50.0	58.9	57.9	302-303 bp	1.0	73.2