Hotspot

ndhF-rpl32

SSC · 113443-114661 · 1219 bp

Back to Debregeasia

Pi 0.00438

MarkerSeek score 61.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1219
variable_sites: 8
pi: 0.00438
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATCTTTTGTTGCC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.0	57.8	1428-1461 bp	1.0	79.0
ndhF-rpl32_p2	CCACATACGGCGAAGATCTT	AAAAGGCTTTCAATGCTGCC	50.0	45.0	57.8	57.8	1436-1469 bp	1.0	77.3
ndhF-rpl32_p3	CGGCGAAGATCTTTTGTTGC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.4	57.8	1429-1462 bp	1.0	76.0
ndhF-rpl32_p4	GCGAAGATCTTTTGTTGCCG	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.4	57.8	1427-1460 bp	1.0	76.0
ndhF-rpl32_p5	GGCGAAGATCTTTTGTTGCC	GCTTTCAATGCTGCCCAATA	50.0	45.0	58.0	57.0	1423-1456 bp	1.0	72.6