Hotspot

psaA-ycf3

LSC · 42256-42998 · 743 bp

Back to Debregeasia

Pi 0.0045

MarkerSeek score 62.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 5
pi: 0.0045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	858-866 bp	1.0	79.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.9	57.8	842-850 bp	1.0	78.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.9	57.8	841-849 bp	1.0	78.7
psaA-ycf3_p4	GAGAAATGACCTGGTCTGGC	AGCGCAGAATTGGTTGAAGA	55.0	45.0	58.3	58.1	876-884 bp	1.0	78.0
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	50.0	50.0	57.9	58.3	886-894 bp	1.0	77.5