Hotspot

trnT-GGU-psbD

LSC · 31857-33178 · 1322 bp

Back to Debregeasia

Pi 0.00454
MarkerSeek score 42.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1322
variable_sites
9
pi
0.00454
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1464-1490 bp 1.0 80.4
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1467-1493 bp 1.0 80.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG AGTAGACCGGACCAACCTAC 55.0 55.0 58.1 58.2 1490-1516 bp 1.0 79.2
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CAGTAGACCGGACCAACCTA 55.0 55.0 58.1 58.2 1491-1517 bp 1.0 79.2
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA AGTAGACCGGACCAACCTAC 50.0 55.0 58.1 58.2 1493-1519 bp 1.0 79.0