Hotspot

trnE-UUC-trnT-GGU

LSC · 31132-31784 · 653 bp

Back to Debregeasia

Pi 0.00306

MarkerSeek score 60.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 3
pi: 0.00306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	740-755 bp	1.0	79.1
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	738-753 bp	1.0	79.1
trnE-UUC-trnT-GGU_p3	TCCTTTCGTAGTCCCCTACC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	797-812 bp	1.0	79.1
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	719-734 bp	1.0	78.7
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	717-732 bp	1.0	78.7