Hotspot

trnC-GCA-petN

LSC · 28114-29021 · 908 bp

Back to Debregeasia

Pi 0.0022

MarkerSeek score 60.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 3
pi: 0.0022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1030-1031 bp	1.0	78.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	974-975 bp	1.0	78.4
trnC-GCA-petN_p3	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	956-957 bp	1.0	77.6
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1091-1092 bp	1.0	77.0
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1035-1036 bp	1.0	76.8