Hotspot

trnS-GCU-trnG-UCC

LSC · 8458-9137 · 680 bp

Back to Debregeasia

Pi 0.00393

MarkerSeek score 61.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 4
pi: 0.00393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TAATCAACTCAAGGGACCCC	50.0	50.0	58.0	56.5	788-791 bp	1.0	68.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TAATCAACTCAAGGGACCCC	50.0	50.0	58.0	56.5	787-790 bp	1.0	68.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TAATCAACTCAAGGGACCCC	50.0	50.0	58.2	56.5	842-845 bp	1.0	67.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	TTAATCAACTCAAGGGACCCC	50.0	47.6	58.0	57.3	789-792 bp	1.0	66.8
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	TTAATCAACTCAAGGGACCCC	50.0	47.6	58.0	57.3	788-791 bp	1.0	66.8