Hotspot

psbK-psbI

LSC · 7622-8108 · 487 bp

Back to Debregeasia

Pi 0.00548

MarkerSeek score 36.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 487
variable_sites: 4
pi: 0.00548
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	612-613 bp	1.0	79.5
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	613-614 bp	1.0	79.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	607-608 bp	1.0	79.5
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	608-609 bp	1.0	79.5
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTACGCCCCGGATCATTAGA	45.0	50.0	58.1	58.3	614-615 bp	1.0	77.7