Hotspot

rps16

Genome · 3630-4681 · 1052 bp

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Pi 0.197

MarkerSeek score 83.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: Genome
length_bp: 1052
variable_sites: 192
pi: 0.197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCCTTCCCCTCCTTTTTCAA	GACTTCTGAAGCTGAGTCGG	50.0	55.0	58.0	58.0	1592 bp	0.5	59.8
rps16_p2	GCCTTCCCCTCCTTTTTCAA	GGACTTCTGAAGCTGAGTCG	50.0	55.0	58.0	58.0	1593 bp	0.5	59.8
rps16_p3	GCCTTCCCCTCCTTTTTCAA	AGGGGATAAGCACCAAGTCT	50.0	50.0	58.0	58.0	1641 bp	0.5	59.7
rps16_p4	GCCTTCCCCTCCTTTTTCAA	CTTTGTGAGGGGGAAAACCA	50.0	50.0	58.0	57.9	1553 bp	0.5	59.3
rps16_p5	GCCTTCCCCTCCTTTTTCAA	TTGTGAGGGGGAAAACCAAG	50.0	50.0	58.0	57.9	1551 bp	0.5	59.3