Hotspot

trnN-GUU-ycf2

Genome · 129788-130305 · 518 bp

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Pi 0.12

MarkerSeek score 82.80

Primer available yes

Label trnN-GUU-ycf2

Feature Metadata

label_name: trnN-GUU-ycf2
feature_type: igs
region: Genome
length_bp: 518
variable_sites: 59
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf2_p1	GATTTACCAATCCCGCCCTT	GGAATATCCGGCGACTCATC	50.0	55.0	57.9	58.0	1711 bp	0.5	59.4
trnN-GUU-ycf2_p2	GATTTACCAATCCCGCCCTT	TCGCTTTGATTCCTACGTGG	50.0	50.0	57.9	58.0	1310 bp	0.5	59.4
trnN-GUU-ycf2_p3	TCGTGTTCATTTCTCCCGAC	GGAATATCCGGCGACTCATC	50.0	55.0	57.9	58.0	1654 bp	0.5	59.4
trnN-GUU-ycf2_p4	TGAATCCCCTGCAACTTTCC	GGAATATCCGGCGACTCATC	50.0	55.0	58.1	58.0	1234 bp	0.5	59.4
trnN-GUU-ycf2_p5	TCGTGTTCATTTCTCCCGAC	TCGCTTTGATTCCTACGTGG	50.0	50.0	57.9	58.0	1253 bp	0.5	59.4