Hotspot

ycf2-ndhF

Genome · 103974-107555 · 3582 bp

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Pi 0.221

MarkerSeek score 56.60

Primer available yes

Label ycf2-ndhF

Feature Metadata

label_name: ycf2-ndhF
feature_type: igs
region: Genome
length_bp: 3582
variable_sites: 116
pi: 0.221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.15
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-ndhF_p1	CGATTGGTGTTCACGCTAGA	CTATCCTGGTGGAAAAGGGC	50.0	55.0	58.0	57.9	1376 bp	0.5	59.5
ycf2-ndhF_p2	TCGATTGGTGTTCACGCTAG	CTATCCTGGTGGAAAAGGGC	50.0	55.0	58.0	57.9	1377 bp	0.5	59.5
ycf2-ndhF_p3	GAGCCTTATCCTGTTTCCGG	CTATCCTGGTGGAAAAGGGC	55.0	55.0	58.0	57.9	1200 bp	0.5	59.2
ycf2-ndhF_p4	CCCACTCTATGTTTACGGCC	CTATCCTGGTGGAAAAGGGC	55.0	55.0	58.1	57.9	1066 bp	0.5	59.1
ycf2-ndhF_p5	TGGATGGGGACGCATTAAAG	CTATCCTGGTGGAAAAGGGC	50.0	55.0	57.9	57.9	1420 bp	0.5	59.1