Hotspot

psbE-petL

Genome · 60841-61588 · 748 bp

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Pi 0.151

MarkerSeek score 82.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 748
variable_sites: 109
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCGTTCTCCTGTATTCCCAG	TCCAGCTAGGGTGATTGGAA	55.0	50.0	58.1	58.0	1085-1102 bp	1.0	89.4
psbE-petL_p2	TAGCTGATATGGACCCCAGG	TCCAGCTAGGGTGATTGGAA	55.0	50.0	58.0	58.0	1428-1445 bp	1.0	89.1
psbE-petL_p3	TAGCTGATATGGACCCCAGG	CCAATTGATCACCGCGTCTA	55.0	50.0	58.0	58.1	1474-1491 bp	1.0	88.8
psbE-petL_p4	CCTATCACAAGTGTACCGGC	TCCAGCTAGGGTGATTGGAA	55.0	50.0	58.1	58.0	1770-1787 bp	1.0	88.5
psbE-petL_p5	ATATTCGTTTGGGCGAGGAC	TCCAGCTAGGGTGATTGGAA	50.0	50.0	58.1	58.0	1226 bp	0.5	59.3