Hotspot

petA-psbJ

Genome · 59661-60073 · 413 bp

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Pi 0.253

MarkerSeek score 81.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 413
variable_sites: 101
pi: 0.253
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTGAAAAAGTGCAGCTGGC	TGGGGTCGTCTCTTCGATAA	45.0	50.0	58.1	57.9	469-727 bp	1.0	88.3
petA-psbJ_p2	TTTGAAAAAGTGCAGCTGGC	TTGGGGTCGTCTCTTCGATA	45.0	50.0	58.1	57.9	470-728 bp	1.0	88.3
petA-psbJ_p3	TTGGCACAGATTTTCCTCGT	TGGGGTCGTCTCTTCGATAA	45.0	50.0	57.7	57.9	505-763 bp	1.0	86.5
petA-psbJ_p4	TTGGCACAGATTTTCCTCGT	TTGGGGTCGTCTCTTCGATA	45.0	50.0	57.7	57.9	506-764 bp	1.0	86.5
petA-psbJ_p5	TGGCACAGATTTTCCTCGTG	TGGGGTCGTCTCTTCGATAA	50.0	50.0	58.5	57.9	504-762 bp	1.0	84.9