Hotspot

psbD-trnT-GGU

Genome · 34264-35623 · 1360 bp

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Pi 0.17

MarkerSeek score 85.70

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1360
variable_sites: 219
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	45.0	55.0	58.1	58.5	1442-1445 bp	1.0	87.5
psbD-trnT-GGU_p2	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	45.0	55.0	57.9	58.5	1441-1444 bp	1.0	87.1
psbD-trnT-GGU_p3	TTTTCCGATGGCAATGGTCA	TGATTTGAGGCCCCCTTAAC	45.0	50.0	58.1	57.5	1459-1462 bp	1.0	86.9
psbD-trnT-GGU_p4	TTTTCCGATGGCAATGGTCA	TTTGAGGCCCCCTTAACTCA	45.0	50.0	58.1	58.5	1456-1459 bp	1.0	86.7
psbD-trnT-GGU_p5	TTTCCGATGGCAATGGTCAT	TGATTTGAGGCCCCCTTAAC	45.0	50.0	57.9	57.5	1458-1461 bp	1.0	86.4