Hotspot

psbK-psbI

Genome · 7205-7757 · 553 bp

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Pi 0.245

MarkerSeek score 84.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 553
variable_sites: 123
pi: 0.245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCCGCAGTTAGCTTTCGATA	AGACAAATAGTTTGAGGGTAAGCA	50.0	37.5	58.4	58.1	555-599 bp	1.0	55.6
psbK-psbI_p2	GCCGCAGTTAGCTTTCGATA	TGCATAGACAAATAGTTTGAGGGT	50.0	37.5	58.4	58.2	560-604 bp	1.0	55.1
psbK-psbI_p3	GCCGCAGTTAGCTTTCGATA	GACAAATAGTTTGAGGGTAAGCA	50.0	39.1	58.4	56.8	554-598 bp	1.0	54.8
psbK-psbI_p4	TAGCTTTTGTTTGGCAAGCC	AGACAAATAGTTTGAGGGTAAGCA	45.0	37.5	57.5	58.1	572-616 bp	1.0	54.8
psbK-psbI_p5	TAGCTTTTGTTTGGCAAGCC	TGCATAGACAAATAGTTTGAGGGT	45.0	37.5	57.5	58.2	577-621 bp	1.0	54.3