Hotspot

ycf1

SSC · 125359-130830 · 5472 bp

Pi 0.0159

MarkerSeek score 51.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 338
pi: 0.0159
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTGGCACAAATTCTGGGAA	ATGAAGGTGGTATGACCCCA	45.0	50.0	57.6	58.0	643-664 bp	1.0	86.2
ycf1_p2	AAGTGGCACAAATTCTGGGA	ATGAAGGTGGTATGACCCCA	45.0	50.0	57.6	58.0	644-665 bp	1.0	86.1
ycf1_p3	CATTTTGGTCCAGGCCTCAA	ATGAAGGTGGTATGACCCCA	50.0	50.0	58.4	58.0	422-440 bp	1.0	85.5
ycf1_p4	TGGTCCAGGCCTCAATATCA	ATGAAGGTGGTATGACCCCA	50.0	50.0	58.1	58.0	423 bp	0.052632	41.1
ycf1_p5	TGGTCCAGGCCTCAATATCA	TGAAGGTGGTATGACCCCAA	50.0	50.0	58.1	58.3	422 bp	0.052632	39.2