Hotspot

rps15-ycf1

SSC · 124963-125358 · 396 bp

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Pi 0.0302
MarkerSeek score 67.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
396
variable_sites
43
pi
0.0302
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GAATCTCCAACTGGCCGATT CCCACCCGTTTGTCAGTAAA 50.0 50.0 58.0 58.0 1135-1178 bp 1.0 87.6
rps15-ycf1_p2 GGTGCTGAGGACCCATATTG CCCACCCGTTTGTCAGTAAA 55.0 50.0 58.0 58.0 1304-1347 bp 1.0 87.2
rps15-ycf1_p3 TCCCAACGCGTTACATAAGG CCCACCCGTTTGTCAGTAAA 50.0 50.0 58.0 58.0 1458-1501 bp 1.0 86.6
rps15-ycf1_p4 GACCCCTTGTTGTCTTCGTT CCCACCCGTTTGTCAGTAAA 50.0 50.0 58.0 58.0 944-987 bp 0.947368 86.5
rps15-ycf1_p5 TCCCAACGCGTTACATAAGG TCTAGATCCCACCCGTTTGT 50.0 50.0 58.0 58.1 1465-1508 bp 1.0 86.3