Hotspot

psaJ-rpl33

LSC · 66730-67172 · 443 bp

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Pi 0.0193
MarkerSeek score 66.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
443
variable_sites
37
pi
0.0193
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTTTCCCAGATGCGTTGACA TTTCGAACACAACCGGTACA 45.0 45.0 58.0 57.7 526-540 bp 1.0 85.6
psaJ-rpl33_p2 ATGCGTTGACATTCCCCTTT TTTCGAACACAACCGGTACA 45.0 45.0 58.1 57.7 517-531 bp 1.0 85.1
psaJ-rpl33_p3 ACATATCTTTCCGTGGCACC TTTCGAACACAACCGGTACA 50.0 45.0 58.0 57.7 605-619 bp 1.0 84.5
psaJ-rpl33_p4 TTCCCAGATGCGTTGACATT TTTCGAACACAACCGGTACA 45.0 45.0 57.8 57.7 524-538 bp 1.0 84.2
psaJ-rpl33_p5 TTTCCCAGATGCGTTGACAT TTTCGAACACAACCGGTACA 45.0 45.0 57.8 57.7 525-539 bp 1.0 84.2