Hotspot

ndhC-trnV-UAC

LSC · 50285-51352 · 1068 bp

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Pi 0.0145
MarkerSeek score 70.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1068
variable_sites
74
pi
0.0145
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCAGGCCCTTTGTTAATCGG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.9 58.1 1022-1221 bp 1.0 87.2
ndhC-trnV-UAC_p2 TAATCGGGGCTAAAACTCCG CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.4 58.1 1009-1208 bp 1.0 83.4
ndhC-trnV-UAC_p3 TCAGGCCCTTTGTTAATCGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1007-1206 bp 1.0 82.7
ndhC-trnV-UAC_p4 CCCTTTGTTAATCGGGGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.3 58.1 1017-1216 bp 1.0 82.5
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAC CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.8 58.1 1004-1203 bp 1.0 81.6