Hotspot

trnT-GGU-psbD

LSC · 31149-32555 · 1407 bp

Back to Daucus

Pi 0.026
MarkerSeek score 69.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1407
variable_sites
115
pi
0.026
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCATGGTAAGGCCTAAGTC CCAGAAAACGCGATTGCATT 55.0 45.0 58.0 58.0 1866-1899 bp 0.947368 85.4
trnT-GGU-psbD_p2 GCCATGGTAAGGCCTAAGTC GAAACGGTCCCTACGTAACC 55.0 55.0 58.0 58.0 1504-1537 bp 0.894737 84.9
trnT-GGU-psbD_p3 CAATATAGAGTCGACCGCCG GAAACGGTCCCTACGTAACC 55.0 55.0 58.0 58.0 1667 bp 0.052632 41.8
trnT-GGU-psbD_p4 CAATATAGAGTCGACCGCCG CCAGAAAACGCGATTGCATT 55.0 45.0 58.0 58.0 2029 bp 0.052632 41.7
trnT-GGU-psbD_p5 GAAGAAGAGCCCATTCGGAG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1933 bp 0.052632 41.6