Hotspot

rps2-rpoC2

LSC · 16077-16292 · 216 bp

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Pi 0.0366
MarkerSeek score 71.50
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
216
variable_sites
27
pi
0.0366
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCGGCCTTCATCATCTCTTC TCCATGATACATCAGAACAATCA 55.0 34.8 58.1 55.5 310-325 bp 1.0 51.1
rps2-rpoC2_p2 CGGCCTTCATCATCTCTTCC TCCATGATACATCAGAACAATCA 55.0 34.8 58.1 55.5 309-324 bp 1.0 51.1
rps2-rpoC2_p3 AATGAACTCCGGCCTTCATC TCCATGATACATCAGAACAATCA 50.0 34.8 58.0 55.5 318-333 bp 1.0 50.9
rps2-rpoC2_p4 AAAATGAACTCCGGCCTTCA TCCATGATACATCAGAACAATCA 45.0 34.8 57.7 55.5 320-335 bp 1.0 50.9
rps2-rpoC2_p5 AATGAACTCCGGCCTTCATC TTCCATGATACATCAGAACAATCA 50.0 33.3 58.0 56.2 319-334 bp 1.0 50.9