Hotspot

atpH-atpI

LSC · 13589-14336 · 748 bp

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Pi 0.0255
MarkerSeek score 67.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
748
variable_sites
69
pi
0.0255
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TGCTTTGGCTTTACTCACGT 45.0 45.0 58.0 58.0 1104-1128 bp 1.0 87.1
atpH-atpI_p2 CTCGCGATACCTTCTACAGC TGCTTTGGCTTTACTCACGT 55.0 45.0 58.0 58.0 1194-1218 bp 1.0 86.5
atpH-atpI_p3 ATAAACGGCGAATTACCCCC ATAGGGGAATCCATGGAGGG 50.0 55.0 58.0 57.9 1268-1285 bp 0.947368 85.4
atpH-atpI_p4 ATAAACGGCGAATTACCCCC TGCTTTGGCTTTACTCACGT 50.0 45.0 58.0 58.0 1569-1586 bp 0.947368 84.3
atpH-atpI_p5 ATAAACGGCGAATTACCCCC TATGGTCCCTGGGTTCCTTT 50.0 50.0 58.0 57.9 1712-1729 bp 0.947368 83.6