Hotspot

trnG-TCC-trnR-UCU

LSC · 9783-9935 · 153 bp

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Pi 0.0565
MarkerSeek score 74.80
Primer available yes
Label trnG-TCC-trnR-UCU

Feature Metadata

label_name
trnG-TCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
153
variable_sites
26
pi
0.0565
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.89
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-TCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 202-242 bp 1.0 75.3
trnG-TCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 205-245 bp 1.0 75.1
trnG-TCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 208-248 bp 1.0 74.7
trnG-TCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 211-251 bp 1.0 74.5
trnG-TCC-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 206-246 bp 1.0 72.9